By Markus Montag
One of many fresh advances in assisted replica treatments (ART), more suitable applied sciences for settling on potential oocytes, sperm, and embryos are of fundamental significance. ironically, the most recent advances provided at meetings and symposia are frequently sluggish to develop into a part of the day-by-day regimen in IVF laboratories. Detailing confirmed and constructing innovations, a pragmatic advisor to picking Gametes and Embryos offers a elementary textual content of ready-to-use ARTs that may be applied successfully within the lab. Read more...
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Extra resources for A Practical Guide to Selecting Gametes and Embryos
Absolute asthenozoospermia and ICSI: What are the options? Hum Reprod Update. 2011;17:684–92. 11. Bourne H, Richings N, Liu DY, Clarke GN, Harari O, Baker HW. Sperm preparation for intracytoplasmic sperm injection: Methods and relationship to fertilization results. Reprod Fertil Dev. 1995;7:177–83. Casper RF, Meriano JS, Jarvi KA, Cowan L, Lucato ML. The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril.
Can pentoxifylline improve the sperm motion and ICSI success in the primary ciliary dyskinesia? Arch Gynecol Obstet. 2009;279:213–15. 18. Ebner T, Tews G, Mayer RB, Ziehr S, Arzt W, Costamoling W, et al. Pharmacological stimulation of sperm motility in frozen and thawed testicular sperm using the dimethylxanthine theophylline. Fertil Steril. 2011;96:1331–6. 19. Soares JB, Glina S, Antunes N, Jr, Wonchockier R, Galuppo AG, Mizrahi FE. Sperm tail flexibility test: A simple test for selecting viable spermatozoa for intracytoplasmic sperm injection from semen samples without motile spermatozoa.
Nowadays, most clinics retrieve the follicular fluid (FF) via transvaginal ultrasound guidance  and a thin single-lumen needle (17–21 gauge)  that is connected to a pressure-controlled aspiration pump, thus avoiding flushing of follicles. 1 Collection of follicular fluid (FF). Deep heat block is needed for keeping prewarmed test tubes at an acceptable temperature during the collection of the FF. Be sure also that the transparent plug is properly inserted into the test tube, so that the aspiration pressure can be maintained.
A Practical Guide to Selecting Gametes and Embryos by Markus Montag